A cortisol urine test measures the amount of the steroid hormone cortisol in the urine.

Cortisol is a steroid hormone released from the adrenal gland in response to ACTH, a hormone from the anterior pituitary gland in the brain. Cortisol levels rise and fall during the day. Highest levels occur at about 6 a.m. to 8 a.m. and lowest levels at about midnight.

Cortisol affects many different body systems. It plays a role in:

• Bone
• Circulatory system
• Immune system
• Metabolism of fats, carbohydrates, and protein
• Nervous system
• Stress responses

The test is done to determine if you have increased or decreased cortisol production. Different diseases, such as Cushing's disease and Addison's disease, can lead to either too much or too little production of cortisol. Urine cortisol levels can help to diagnose these conditions.

A 24-hour urine sample is needed. The health care provider will instruct you, if necessary, to stop taking drugs that may affect the test.

• On day 1, urinate into the toilet when you get up in the morning. Afterwards, collect all urine in a special container for the next 24 hours. Keep the container in a cool place during the test period.
• On day 2, urinate into the container when you get up in the morning.
• Cap the container. Label the container with your name, the date, the time of completion, and return it as instructed. Keep it in the refrigerator or a cool place until you return it to the laboratory.

FOR INFANTS

Thoroughly wash the area around the urethra. Open a urine collection bag (a plastic bag with adhesive paper on one end), and place it on the infant. For boys, the entire penis can be placed in the bag and the adhesive attached to the skin. For girls, the bag is placed over the labia. Diaper as usual over the secured bag.

This procedure may take a couple of attempts -- lively infants can displace the bag. Check the infant frequently and change the bag after the infant has urinated into the bag. Drain the urine into the container for transport to the laboratory.

Deliver the urine to the laboratory or your health care provider as soon as possible.

No special preparation is necessary for this test. If you are taking the collection from an infant, you may need a couple of extra collection bags.

The test involves only normal urination, and there is no discomfort.